Cell and Cryobiology Research Group

Cell and Cryobiology Research Group

• D Lermen, B Blömeke, R Browne, A Clarke, P W Dyce, T Fixemer, G R Fuhr, W V Holt, K Jewgenow, R E Lloyd, S Lötters, M Paulus, G McGregor Reid, D H Rapoport, D Rawson, J Ringleb, O A Ryder, G Spörl, T Schmitt, M Veith and P Müller (2009). Cryobanking of viable biomaterials: implementation of new strategies for conservation purposes. Meeting Review. Molecular Ecology (2009) 18, 1030–1033.
  

• Molecular Ecology [external link to abstract]

• C Lin, E Spikings, T Zhang and D M Rawson (2009) Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres. Cryobiology, 59(1):42-7.
• C-H Lin, E Spikings, T Zhang and D M Rawson (2009). Housekeeping genes for cryopreservation studies on zebrafish embryos and blastomeres. Theriogenology, 71(7),1147-1155.

• Theriogenology [external link to abstract]

• C-H Lin, T Zhang and D M Rawson (2009). Cryopreservation of zebrafish (Danio rerio) blastomeres by controlled slow cooling. CryoLetters, 30(2), 132-141.

• S Tsai, D M Rawson and T Zhang (2009). Studies on chilling sensitivity of early stage zebrafish (Danio rerio) ovarian follicles. Cryobiology, 58(3), 279-286.

• S Tsai, D M Rawson and T Zhang (2009). Development of cryopreservation protocols for early stage zebrafish (Danio rerio) ovarian follicles using controlled slow cooling. Theriogenology, 71(8), 1226-1233.

• T Zampolla, E Spikings, T Zhang, D M Rawson (2009). Effect of methanol and Me2SO exposure on mitochondrial activity and distribution in stage III ovarian follicles of zebrafish (Danio rerio). Cryobiology 59(2), 188-194.

• T Zampolla , T Zhang , W V Holt and D M Rawson (2009). Distributional arrangement of mitochondria in the granulosa cells surrounding stage III zebrafish (Danio rerio) oocytes. Theriogenology 72(1), 111-119.

• M Guan, D M Rawson and T Zhang (2008). Cryopreservation of zebrafish (Danio rerio) oocytes using improved controlled slow cooling protocols. Cryobiology, 56, 171-256.

• S Tsai, D M Rawson and T Zhang (2008). Studies on cryoprotectant toxicity to early stage zebrafish (Danio rerio) ovarian follicles. CryoLetters, 477-483.

• T Zhang, D M Rawson, L Tosti and O Carnevali (2008). Cathepsin activities and membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196^oC using controlled slow cooling. Cryobiology, 56, 138-143.

• Y Ding, T Zhang and D M Rawson (2008). Mediated amperometric whole-cell biosensors for rapid in situ screening of heavy metal contaminated soil. In Luo et al (eds). Proceedings of 3rd International Conference on Soil Pollution and Remediation, 209-215.

• T Zampolla, T Zhang and D M Rawson (2008). Evaluation of zebrafish (Danio rerio) ovarian follicle viability by simultaneous staining with fluorescein diacetate and propidium iodide. CryoLetters, 29, 463-475.

• R Wang, M Guan, D M Rawson and T Zhang (2007). Ultrasound enhanced methanol penetration of zebrafish (Danio rerio) embryos measured by permittivity changes using impedance spectroscopy. European Biophysics Journal, 37, 1039-1044.

• M Guan, D M Rawson and T Zhang (2007). Development of a new method for isolating zebrafish oocytes (Danio rerio) from ovaries mass. Theriogenology, 69, 269-275.

• C Lin, T Zhang, G Elgar and D Rawson (2007). Initial studies on cryopreservation and gene expression in isolated blastomeres of zebrafish (Danio rerio). Cryobiology, 55, 365.

• T Zhang, D M Rawson, I Pekarsky, I Blais and E Lubzens (2007). Low temperature preservation of fish gonad cells and oocytes. In P. J. Babin, J. Cerda and E Lubzens (Eds) 'The Fish Oocyte: From Basic Studies to Biotechnological Applications', Kluwer Academic Publishers B.V., 411-436.
• J Kopeika, E Kopeika, T Zhang and D M Rawson (2006). Cryopreservation of sperm of loach (Misgurnus fossilis). In M.P. Herraez, V. Robles and E Cabrita (Eds) ‘Protocols for the cryopreservation of sperm from marine and freshwater species’. CRC Press, 323-328.
• R Y Wang, T Zhang, Q-Y Bao and D M Rawson (2006). Study on fish embryo responses to the treatment of cryoprotective chemicals using impedance spectroscopy. European Biophysics Journal, 35, 224-230.

• J Kopeika, D M Rawson and T Zhang (2006). Preliminary study on modification of yolk sec of zebrafish embryos (Danio rerio) using microinjection. CryoLetters, 27, 319-328.

• T Zhang, R Y Wang, Q-Y Bao and D M Rawson (2006). Development of a new rapid measurement technique for fish embryo membrane permeability studies using impedance spectroscopy. Theriogenology, 66, 982-988.

• H Wex, D Rawson. and T Zhang. (2005). Use of biosensor and impedance spectroscopy assays to investigate the influence of temperature on E. coli sensitivity to 3,5-dichlorophenol. Bioelectrochem. Acta, 51, 5157-5162.
• T Zhang, D M Rawson and G Elgar (2005). Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 570, 49 – 61.
• T Zhang, H Wex, D M Rawson and R S Thompson (2005). Rapid ecotoxicological testing using transformed BF-2 cell lines incorporating luminescent reporter genes. Toxicology in vitro, 19(6), 797-803.
• T Zhang, A Isayeve, S L Adams and D M Rawson. Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants. Cryobiology, 50, 285-293.
• S L Adams, T Zhang and D M Rawson. The effect of external medium composition on membrane water permeability of zebrafish (Danio rerio) embryos. Theriogenology, 64,1591-1602.
• J Kopeika, T Zhang, D M Rawson and G Elgar. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio) blastomere cells. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 570, 49 – 61, 2005.
• A Isayeva, T Zhang and D M Rawson. Studies on chilling sensitivity of zebrafish (Danio rerio) oocytes. Cryobiology, 49, 114-122, 2004
• M Plachinta, T Zhang and D M Rawson. Studies on cryoprotectant toxicity to zebrafish (Danio rerio) oocytes. CryoLetters, 25, 415-424, 2004
• T Zhang. Cryopreservation of gametes and embryos of aquatic species. In B Fuller, N Lane and E Benson (Eds) Life in the Frozen State, Taylor and Francis, London, 415-435, 2004.

• Life in the Frozen State

• J Kopeika, E Kopeika, T Zhang, D M Rawson and W V Holt. Effect of DNA repair inhibitor (3-aminobenzamide) on genetic stability of loach(Misgurnus fossilis) embryos derived from cryopreserved sperm. Theriogenology, 61, 1661-1673, 2004.
• J Kopeika. The effect of cryopreservation on the genome of fish reproductive cells. PhD thesis. University of Luton, 2003.
• J Kopeika, T Zhang, D M Rawson and G Elgar. Effect of cryopreservation on mitochondrial DNA of zebrafish (Danio rerio)blastomere cells. Cryobiology, 47, 269-270, 2003.
• T Zhang, M Plachinta, A Isayeva and D M Rawson. Studies on zebrafish (Danio rerio) oocytes sensitivity to chilling and cryoprotectant toxicity. Cryobiology, 47, 259, 2003.
• J Kopeika, E Kopeika, T Zhang and D M Rawson. Studies on the toxicity of dimethyl sulfoxide, ethylene glycol, methanol and glycerol to loach (Misgurnus fossilis) sperm and the effect on subsequent embryo development. Cryoletters, 24, 365-374, 2003.
• J Kopeika, E Kopeika, T Zhang, D M Rawson and W V Holt. Detrimental effect of loach (Misgurnus fossilis) sperm cryopreservation on subsequent embryo development are reversed by incubating fertilised eggs in caffeine. Cryobiology, 46, 43-52, 2003.
• T Zhang, X-H Liu and D M Rawson. Effects of methanol and developmental arrest on chilling injury in zebrafish (Danio rerio) embryos. Theriogenology, 59, 1545-1556, 2003.
• T Zhang and D M Rawson. Cryopreservation of luc gene transfected bluegill sunfish fibroblast cell line. Cryo-Letters, 23, 191-196, 2002.
• R Billard and T Zhang. Techniques of genetic resource banking in fish. pp.156-170. In: P F Watson and W V Holt (eds.) Cryobanking the genetic resource. Wildlife conservation for the future? Taylor and Francis, London, 2001.
• J Kopeika, T Zhang, E Kopeika, W V Holt and D M Rawson. Indirect evidence of genetic effect of cryopreservation on the sperm of loach (Misgurnus fossilis). Cryobiology, 43, 338, 2001.
• X-H Liu, T Zhang and D M Rawson. Differential scanning calorimetry studies of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. Journal of Experimental Zoology, 290, 299-310, 2001.
• T Zhang, A Bentley and D M Rawson. Cryopreservation of luc gene transfected bluegill sunfish fibroblasts cell line. Cryo-Letters, 22, 81-82, 2001.
• X-H Liu, T Zhang and D M Rawson. Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Dani rerio) embryos. Theriogenology, 55, 1719-1731, 2001.
• X-H Liu, T Zhang and D M Rawson. Differential scanning calorimetry study on the characterisation of intraembryonic freezing and cryoprotectant penetration in zebrafish (Danio rerio) embryos. Cryobiology, 41, 355-356, 2000.
• D M Rawson, T Zhang, D Kalicharan and W L Jongebloed. FE-SEM and TEM studies of the chorion, plasma membrane and syncytial layers of the gastrula stage embryo of the zebrafish (Brachydanio rerio): a consideration of the structural and functional relationship with respect to cryoprotectant penetration. Aquaculture Research, 31, 325-336, 2000.
• X-H Liu. Studies on limiting factors relating to the cryopreservation of fish embryos. PhD Thesis, University of Luton, March 2000.
• X-H Liu, T Zhang and D M Rawson. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39, 318-319,1999.
• X-H Liu, T Zhang and D M Rawson. The effect of partial removal of yolk on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryobiology, 39, 236-242, 1999.
• X-H Liu. Studies on limiting factors relating to the cryopreservation of fish embryos. PhD Thesis, University of Luton, March 2000.
• X-H Liu, T T Zhang and D M Rawson. Effects of cooling rates and anoxia on the chilling sensitivity of zebrafish (Danio rerio) embryos. Cryo-Letters, 19, 309-318, 1998.
• D Kalicharan, W L Jongebloed, D M Rawson and T T Zhang. Variation in fixation techniques for field emission SEM and TEM of zebrafish (Brachydanio rerio) embryo inner and outer membranes. J. Electron. Microsc., 47(6), 645-658, 1998.
• I Stokroos, D Kalicharan, D M Rawson and T T Zhang. Non-coating and cryo- FE-SEM of zebrafish (Brachydanio rerio) embryo blastodermal cells and syncytial layer. Proc. Dutch Soc. for Microscopy, 98, 100-102, 1998.
• X-H Liu, T T Zhang and D M Rawson. Feasibility of vitrification of zebrafish (Danio rerio) embryos using methanol. Cryo-Letters, 19, 309-318, 1998.
• T T Zhang and D M Rawson. Permeability of dechorionated 1-cell and 6-somite stage zebrafish (Brachydanio rerio) embryos to water and methanol. Cryobiology, 32, 239-246, 1998.
• T T Zhang, D M Rawson and W L Jongebloed. Further studies on zebrafish (Brachydanio rerio) embryo membranes: permeability and ultra-structure. Cryo-Letters, 3, 65, 1998.
• D Kalicharan, W L Jongebloed, T T Zhang and D M Rawson. The zebrafish (Brachydanio rerio) egg/embryo inner and outer membrane: A FE-SEM and TEM investigation. Part 1: Chorion. In: Belendsen W (ed) Jaarboek NVvM'97. Karstens Drukker, Leiden, 96-98, 1997.
• W L Jongebloed, D Kalicharan, T T Zhang and D M Rawson. The zebrafish (Brachydanio rerio) egg/embryo inner and outer membrane: A FE-SEM and TEM investigation. Part 2: Fixation regimes. In: Berendsen W (ed) Jaarboek NVvM'97, Karstens Drukker, Leiden, 94-96, 1997.
• X-H Liu, T T Zhang and D M Rawson. A preliminary study on vitrification of intact zebrafish (Brachydanio rerio) embryos using methanol. Cryobiology, 35(4), 372, 1997.
• D M. Rawson, T T Zhang and W Jongebloed. Structure-permeability relationships of the chorion and vitelline membranes of zebrafish (Brachydanio rerio) embryos. Cryo-Letters, 18(2), 67, 1997.
• T T Zhang, G Maisse and D M Rawson. Preliminary studies on the cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.). Cryo-Letters, 17(6), 303-308, 1996.
• T T Zhang and D M Rawson. Permeability of the vitelline membrane of zebrafish (Brachydanio rerio) embryos to methanol and propane-1,2-diol. Cryo-Letters, 17(6), 273-280, 1996.
• T T Zhang and D M Rawson. Feasibility studies on vitrification of zebrafish (Brachydanio rerio) embryos. Cryobiology, 33, 1-13, 1996.
• T T Zhang and D M Rawson. Preliminary studies on vitelline membrane permeability of zebrafish (Brachydanio rerio) embryos. Cryo-Letters, 16, 319, 1995.
• T T Zhang and D M Rawson. Studies on chilling sensitivity of zebrafish (Brachydanio rerio) embryos. Cryobiology, 32, 239-246, 1995.
• T T Zhang. Investigations into the cryopreservation of zebrafish (Brachydanio rerio) embryos. PhD Thesis, University of Luton, 1994.
• T T Zhang and D M Rawson. Cryoprotectant toxicity and permeability studies on zebrafish (Brachydanio rerio) embryos. Cryobiology, 6, 640, 1993.
• T T Zhang, D M Rawson and G J Morris. Cryopreservation of pre-hatch embryos of zebrafish (Brachydanio rerio). Aquatic Living Resources, 6, 145-153, 1993.

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